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Minor query 6: Thanks for this suggestion.
Minor query 1 We have used all the members from PASS2 for all the sequence searches.
Minor query 4: We are sorry about this and we have now modified Figure 2 in the revised manuscript.
Minor query: In the next paragraph, you report that Table 1 shows 41% of the 4361 100%-similarity sequences belong to the L functional category another 41% belong to the unknown function category.
Minor query 1: It is difficult to obtain patterns qualifying stringent cut-offs followed (please refer to Methods section) when the sequence identity is reduced to 40% that will be applicable for all the superfamilies.
Minor query 2 If a pattern is >15 amino acids in length, the long pattern is broken into subsets of patterns which qualify all the stringent cut-offs and have a maximum length of 15.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com