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Focusing on an N-terminally truncated (Δ108) variant of ST6Gal-I previously shown to represent a minimally sized, still active form of ST6Gal-I, we show here that protein expression engineering and optimization of bioreactor cultivation of P. pastoris KM71H (pPICZαB) synergized to enhance the maximum enzyme titer about 57-fold to 17 units/L.
These simple, minimally sized homes provided housing for predominately white families working in Oak Ridge during the Project years.
This indicates that lytic granules are closely associated with F-actin and thus secreted through minimally sized clearances.
Intraprocedural CT scans of the thorax showed no air within the pleural cavity following the first ablation and a minimally sized residual pneumothorax following the second ablation, which required no subsequent intervention or further treatment.
A key feature of our approach is that it provides as output a minimally sized set of genes that captures the genes which harbor causal variants with probability ρ.
These efforts used multiple high resolution imaging techniques to demonstrate that the actin network does not act as a barrier to secretion, but instead enables the secretion of lytic granules through minimally sized clearances.
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In order to meet the design goals of this study, Au NCs needed to be composed of small Au NPs of the minimally required size for detection by OCT and DvOCT.
To obtain a better estimate on the minimally required size of input DNA, we analysed randomly generated sequences of increasing length (Fig. 3).
We chose a minimally important effect size of 0.4 and a sample size of 50 per group as being the best balance of resource use and protection against Type II error, assuming β=0.8 and α=0.05.
Thus, which benefits are appropriate for an individual participant depends, minimally, on the size of the social benefits created and size of the group of participants who have each borne a substantial net risk, and perhaps also the participant's average or actual contribution.
Our sample size calculation was based on the RDQ index as the primary endpoint with a minimally detectable effect size determined as a difference of 0.4 standard deviations (SD) based on published data [ 68, 77, 78].
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CEO of Professional Science Editing for Scientists @ prosciediting.com