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Mice with a K14-Cre/Smoflox/flox mice were produced as described by Gritli-Linde et al. [33].
ALDH2 transgenic mice were produced using chicken β-actin promoter.
ALDH2 transgenic mice were produced using the chicken β-actin promoter.
Adult cd81 mice were produced by crossing two inbred strains, NIHS-BC/Tac and 129X1/SvJ, carrying the cd81 mutation as heterozygotes.
RNF13-/ and RNF13+/+ mice were produced by crossing RNF13+/- heterozygous mice.
But the strategy is far from perfect: Only seven mice were produced from 210 fertilization attempts, and they all died at a young age.
Knock-in mice were produced by homologous recombination.
Chimeric mice were produced and germline transmission was achieved.
M5R−/− mice were produced as described previously [9].
A total of 14 chimeric mice were produced.
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However, only a fewer iPS-mice were produced compared with wild-type R1 ESCs (Fig. 1D).
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