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Mice were derived from breeding of R6/1 hemizygous male mice, obtained from a colony at the Howard Florey Institute (Melbourne, Australia), with WT strain-matched B6CBAF1/J females.
Mice were derived from a screen initially conduced by Gong and colleagues [ 23].
Bone marrow-derived macrophages from BALB/c, C57BL/6 or NZB mice were derived and cultured as described [ 91].
Human lymphocytes in humanized mice were derived using stem cells from 5 different human donors, and isolated from humanized mice spleen 3 days after irradiation.
Given that MBP is not only expressed in oligodendrocyte myelin but also Schwann cell myelin47, we determined whether the new myelin produced in Myrf ICKO mice were derived from Schwann cells.
The Afh (after hours) mice were derived at the Medical Research Council MRC Mammalian Genetics Unitit (Harwell, UK) from an ENU mutagenesis program, using ENU mutagenized BALB/c males which were crossed to C3H/HeH females.
Twelve weeks after the transplant, about 10 15% of mature blood cells in recipient mice were derived from donor HSCs, whereas there was no detectable donor HSC engraftment in recipients fed a complete diet.
Transgenic mice were derived that constitutively express a trans-dominant-negative kinase-defective mutant PKR under control of the beta-actin promoter.
Rac1 cKO mice were derived from two different Cre (Le-Cre and MLR-10) transgenic mice in which lens-specific Cre expression starts at embryonic day 8.75 and 10.5, respectively, in both the lens epithelium and fiber cells.
Nrf2−/−/HLL mice were derived in a similar fashion.
p53−/−p73+/− mice were derived from crosses between the double heterozygous animals.
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