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Hairlines were made by using the corner of the nib and were nearly invisible in manuscripts.
The tip of the nib is tiny, making it perfect for precision work.
Step Two When you are happy with the broad dimensions of the nib, consider ink supply.
In the latter case, the HV was applied via a Pt wire inserted into the reservoir feature of the nib.
However, to compare the evolution of the full-length TEV and TEV-Δ NIb, we had to devise a setup in which the integrity of the NIb gene is ensured for TEV.
Both inserts (Nib+ and λ-spacer) were subcloned into another pT3T7 plasmid containing a 503 bp HindIII fragment of the 3" coding sequence of the Nib gene (nucleotides 8529-2022) in orientation, in SacI - SphI restriction sites.
Surprisingly, the 5′ start of the genomic deletion was clustered in NIaPro, suggesting a possible recombination hotspot outside of the NIb cistron.
Sand down the edge of the nib so that when you hold the pen in this way, the nib is flush with the paper.
Just keep the nib in the paint and make sure not much paint is settled on the top of the nib.
Dip the pointed end of the nib into the open ink bottle.
Place the lip of a white 260Q balloon over the nib of your pump.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com