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Since the geometric matching alignment and scoring method uses rigid conformations, multiple conformers of each query molecule must be generated.
ProSMART (Procrustes Structural Matching Alignment and Restraint Tool) was used for generation of external restraints for the refinement [ 32].
Of the 19,117 Arabidopsis genes matching alignment assemblies, only 2,867 (15%) lack a FL-cDNA match.
In order to perform matching, alignment of DNA sequences and searching for putative SNPs, the SSAHAsnp Program (Sequence Search and Alignment by Hashing Algorithm) was used [ 65].
As a further test of accuracy, we compare all matching alignment scores against the optimal scores determined by SSEARCH.
Similar(55)
Structural matching, alignments, ribbon figures, capsid predictions and root mean square deviation (RMSD) data were produced using Chimera [55].
All uniquely matching alignments were retained.
BWA-SW produced a sub-optimal score for 1 of 483 786 matching alignments (0.0002%).
For reasons discussed in Section 2, YAHA produced sub-optimal scores for 20 of 522 244 matching alignments (0.0038%).
To check the uniqueness of an oligodeoxyribonucleotide sequence within the whole genome sequence, we sampled random subsequences from the E. coli genome, and searched for their matching alignments throughout the genome.
However, with increase of mismatches (-v) and multiple alignments (-m) in Bowtie1 options, miRNA precursors identified by eRNA covered almost all precursors identified by miRspring and miRDeep2, due to the different considerations of these tools on multiple or non-exactly matching alignments.
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