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The Illumina sequencing technology is the next generation sequencing (NGS) which is a powerful tool utilized in many researching areas, including re-sequencing, micro-RNA expression profiling, DNA methylation, de novo transcriptome sequencing and whole sequencing [ 15- 21], especially in the analysis of whole-genome expression profiling [ 22- 25].
NGS, including the Roche/454 Genome Sequencer FLX Instrument, the ABI SOLiD System and the Illumina Genome Analyser, was a powerful tool and was untilized in many researching areas, including re-sequencing, micro-RNA expression profiling, DNA methylation, especially de novo transcriptome sequencing for non-model organisms [ 5- 13].
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