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They demonstrated how to encrust a pork loin within what was essentially a large crispy pork rind, how to make stewed prunes look like coal and how to make a "constructed cream" — breaking apart a fat and a liquid into tiny droplets and mixing them together into something that had the fluidity of heavy cream.
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However, limited enzyme sites and increased insertion size lower the efficiency to make a construct.
We chose to make a construct containing a GFP gene with 4 introns and 5 exons (the same construct as described previously [21] but with introns).
A major aim in this initial work was to make a construct that was highly responsive to oestrogenic EDCs.
To make a construct including the 3.0-kb region of the AtNCED3 promoter fused with the GUS gene, the promoter region of Arabidopsis genomic DNA and the GUS-NOS terminator region of the pBI101 vector were amplified by PCR.
We made a construct containing eight anthocyanin-related genes (two regulatory genes from maize and six structural genes from Coleus) driven by the endosperm-specific promoters,plus a selectable marker and a gene for marker excision.
To test this hypothesis, we made a construct coding for a non-HA tagged version of the 38 aa TLR4 transmembrane domain.
To test the first hypothesis, we made a construct expressing the peptide from Arg to Asp on human α1 as a GST fusion.
We then made a construct that expresses only the first 640 amino acids of CSPG4 (CSPG4-N1-640, Figure 3A), and the Fc-tagged CSPG4-N30-640 was pusinged using protein G agarose.
Grover and Roughley [ 55] made a construct containing the Col2a1 promoter driving rtTA that undergoes a conformational change, allowing it to bind to the tetO element that was present within the same construct, coupled to a minimal cytomegalovirus (CMV) promoter.
We made a construct containing the full Saccharomyces cerevisiae Smc3 ATPase domain with the coiled coils and hinge replaced by a short flexible linker (Supplementary Figures 2c and d) to create a monopartite ATPase head (ScSmc3AH).
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