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The major technical difficulty both in the original version of the procedure and its adaptation in our work is negative selection of bacterial clones with resolved plasmid-chromosome co-integrates.
Although the method is developed for complex quantitative traits with discrete phenotypes, it would not involve major technical difficulty to extend the ideas and principles behind the newly developed method to cope with continuous phenotypes.
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Thanks for your Nov. 3 editorial "Major Technical Difficulties," warning of federal law updates requiring phone and broadband carriers to ensure that their networks can be wiretapped.
The production may not be electrifying but it does offer a very enjoyable short, sharp theatrical shock, even when struck at its climax by major technical difficulties at the press performance yesterday afternoon.
Sure, Kinect 2.0 solves one of the major technical difficulties: the need for masses of space in front of the TV (the new 1,080p camera with wider lens can cope with more people, and those people can be closer to the telly), but what of the games?
The proposed model, along with the associated wavelet-based solution procedure, is intended to overcome major technical difficulties which are specific to ABH structures: the non-uniform wavelength distribution and ABH-induced wave compressions at the high frequency range in a realistic structure of finite size.
The procedure could be performed without any major technical difficulties and no nerve injury was observed.
Here, we studied up to seven patients per day and there were no major technical difficulties or patient-dependent interruptions of the investigation.
Nevertheless, despite the major technical difficulties and frequent invalid results, the assay was highly sensitive in the limited number of evaluable results.
The remaining 51 patients presented no major technical difficulties and experienced no significant adverse events.
Comparing with environmental robustness, genetic robustness, on the other hand, is connected with major technical difficulties [ 20].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com