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The increase in m-to-s flux is small, however, it should be noted that the incubations time with AII was limited due to the limited viability of mouse jejunum in Ussing chambers.
The use of primary human astrocytes from central nervous system tissue is extremely difficult and is limited by its high sensitivity and limited viability.
A significant limitation has been the limited viability of allogeneic cells used in the majority of tissue engineered skin equivalents and the high cost and limited shelf life associated with using autologous cells.
Furthermore, hAMSCs cultured in standard conditions have shown limited proliferative and migratory capabilities, as well as limited viability.
However, assessing secondary neurosphere formation was precluded because very few spheres were generated in the knockdown groups in the primary assay and the neurospheres had limited viability.
Since electron microscopy showed no ORF75c-dependent virion assembly defect, the limited viability of ORF75c− viruses seemed likely to reflect a problem in establishing infection.
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To date, it is still difficult to define the extent to which allogenic/heterogenic MSCs contribute to tissue regeneration, because immune rejection driven by recipient CD4+ and/or CD8+ T lymphocytes and oxidative stress within injured areas were reported to limit viability of infused MSCs.
As a result of this reduction limited the viability possible to achieve post-concentration, as the final viabilities were below the target by 15 20%.
In the present contribution, an improvement in memory reduction was carried out, which limited accelerator viability usage.
Various in vitro liver models like primary human hepatocytes, immortalized human hepatic cell lines, liver slices and microsomes have been used; but limited by viability, hepatic gene expression and function.
This limited the viability of the imaging preparation to a few hours, corresponding to a single behavioral session.
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