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However, SPECT has limitations in quantification of radiotracer uptake, the speed of dynamic imaging, spatial resolution, and tissue attenuation.
Despite limitations in quantification and prospective analysis of MSC, SA-β-gal is the most widely used biomarker for senescent and aging cells.
Again we detected around 90% co-localisation, so it is possible that the residual 10% can be accounted for by limitations in quantification rather than a biologically significant pool of GPI-anchored proteinternalisedseparatelytely from transferrin.
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Here, we describe a new method that circumvents the above-mentioned limitation in quantification through homogenization of the organs and dilution of the tissue lysates in order to infer in the linear range of fluorescence.
He highlighted the limitations in the quantification and comparison of consumption levels, such as the delay that may occur between data collection and consumption point.
The limitation in particle quantification thus is related to the electron beam penetration and excitation volume from the underlying substrate.
Nevertheless, despite this drawback, fluorescein is the most widely used fluorescent tag due to its low cost, and these limitations in the absolute quantification based solely on fluorescence intensity are rarely taken into account.
However, due to the limitations in scalability and quantification of western blotting, the optimized RPPA was applied to profile these signaling pathways within TNFR1 expressing cells.
However, limitations in reproducibility and quantification have led to the development of the powerful two-dimensional differential gel electrophoresis (2D-DIGE) [ 36, 37].
However, these methods contain inverse algorithms to integrate dynamic data and have limitations in their uncertainty quantifications on new production wells.
The slight discrepancy between copy number and the percentage of pSTAT-5 expressing pSTAT-5 expressingly due to limitations in the acellscy of quantisication by FACS and QPCR.
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