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For analysis of neuronal or astrocyte differentiation, passage 5 cells were switched to differentiation media (same as proliferation media except with 2% FCS and no EGF or bFGF) and let proceed for the specified time.
The cleavage reaction was started by adding substrate in fivefold molar excess over the enzyme, and let proceed for 30 min at 37°C, after which it was stopped by the addition of SDS-PAGE sample buffer and transfer on ice.
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Let's proceed.
So let's proceed.
"Let us proceed".
Let's proceed to policy.
"Let him proceed, and work with him".
A fair retort; but let us proceed.
And with that, let's proceed.
"Let's proceed with the debate".
"It's dangerous there," they warned, but let us proceed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com