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Preliminary trials were made to determine optimal concentrations of reagent, and suitable controls lacking substrates were run to correct for non-specific activity.
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Fungal oxidative exo-enzymes lacking substrate specificity play a central role in the cycling of soil organic matter.
In this assay, ArcB, an E. coli HK, added in excess and hence lacking substrate specificity, transfers its phosphate to the phosphoacceptor site in a receiver domain.
Sumoylation or ubiquitination fusion protein methodology is a powerful complementary strategy for mutant approach that is used to discriminate between the consequence of lacking substrate sumoylation and conformational change-related artifacts [ 211- 215].
To overcome the problem of signal drift, control experiments lacking substrate were performed and the measured phase shifts were corrected using the relevant blank recordings (cf. Figures 5, 6 and 7).
However, tissues engineered from completely biological cell sheets lack substrate cues and possess poor mechanical strength.
The PPP1R15B-PP1 binary complex lacks substrate specificity.
Surprisingly, PPP1R15-PP1 binary complexes reconstituted in vitro lacked substrate selectivity.
Most notably, P localities lack substrate of the smaller size classes.
When inorganic phosphate is absent, the phosphate transporter lacks substrate and relays this information through a cascade of three proteins, PhoU, PhoR, and PhoB to cause, among others, the transcription of the phoA gene which encodes alkaline phosphatase.
See section "BiP deletion mutants that lack substrate binding domain attenuate UPR signaling" and Figure 6. 2) Does deletion or alteration of subregion V in Ire1 and Perk affect the binding of their luminal regions to BiP in cells?
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