Exact(36)
In fact, the colony forming assay can only give information about the ability of the cells to proliferate but cannot discriminate the cellular mechanism underlying this lack of proliferation, which in our case appears to be apoptosis in the two control groups with cellular senescence in the MAD2↓ cells.
However, because most proliferating cells grow in size before cell division, the complete lack of proliferation in our model allows separation of growth effects.
PCNA staining confirmed lack of proliferation in late passage cells under static or flow conditions.
A major limitation to cardiac tissue engineering and regenerative medicine strategies is the lack of proliferation of postnatal cardiomyocytes.
Jarvis attributes the lack of proliferation in the home market to the absence of hardware that offered two side-by-side joysticks.
The lack of proliferation of MEFs obtained from E9.5 Sp2null embryos could reflect the developmental retardation of these embryos or could be due to the lack of Sp2.
Similar(24)
Indeed, claudin-low tumors lack expression of proliferation genes and are hence likely to comprise slow-cycling tumors quite unlike the basal-like subtype [ 2].
This is likely an effect of ultrasonic cavitation and is visible thanks to the lack of bacterial proliferation.
Lack of germline proliferation in C. elegans, either by laser ablation of the germ cell precursors or by mutation, causes an increase in lifespan [8], [9].
Cell death and lack of cell proliferation in combination with continued programmed cell shedding at the apices of villi results in extensive depletion of crypt cells, leading to crypt shrinkage and perturbed morphology [35].
Lack of cell proliferation may be attributed to the cumulative time required to remove the cells from the culture surface to put them into suspension, the shear forces exerted on the cells by the FACS machine, and a lack of paracrine signalling required to stimulate cell survival and proliferation.
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