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However, what differentiates active CRISPR Cas systems from inactive CRISPR Cas systems in mammalian cells remains to be understood and currently there is a lack of methodology to utilize inactive CRISPR Cas systems for mammalian genome-editing applications.
Although established technology exists, there is a lack of methodology to construct multiple hardware and software platforms and interoperate them effectively.
There have been some attempts to evaluate the fuel pool size on a flat or longitudinally sloped surface, whereas there is a lack of methodology for evaluation of fuel pool size, fire zone length and fire heat release rate on a sloped roadbed surface.
Due to the lack of methodology for evaluating such systems, a series of measures for the quantitative assessment of the user effort in interactive onset detection schemes have been proposed.
The key problem would-be crowdsourcers face: lack of methodology.
The real problem is the utter lack of methodology or research that has gone into this crusade against flash trading, either by its promoters or enforcers.
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Nevertheless, there are a lack of methodologies to properly support the development process of these systems.
The lack of methodologies to do so using reservoir simulations is the main motivation for this work.
There is a lack of methodologies that helps the designer to identify control system's main sequence, initial conditions, system start-up and shutdown.
Nevertheless there seems to be a lack of methodologies for supporting stakeholders in decisions related to the introduction of green vehicles, whose allocation should be performed since the network design process in order to optimize their available green capacity.
More complex unit operations have largely been unexplored due to complexities interfacing inline analytical equipment to unit operations or a lack of methodologies that can be applied to measure attributes of interest.
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