Exact(1)
Dehydration produces predominantly one Cu II) species located in recessed sites such as hexagonal prism for L and offertite and six-ring window of a gmelinite cage for omega based on a lack of broadening of its ESR lines by added oxygen.
Similar(59)
In this study, a lab-scale tubular type ESP is designed, constructed and tested with the main purpose of broadening the lack of experimental results with this kind of ESP.
There's been a lot of broadening.
As described above, the slow rate of the exchange reaction between free CO and free CO2 (∼500 times slower than the CO exchange broadening) and the lack of CO2 line broadening clearly show that an exchange between CO and solution CO2 is not part of the CO line broadening mechanism.
The ferrylHb species was ruled out because: (a) there was no exposure to H2O2, nor were there any environmental conditions that would lead to such exposure; and, (b) the spectra do not show evidence of its existence, primarily by the lack of spectral broadening where the 577 nm shoulder drops off steeply around 585 nm.
Akin to the wild-type scenario, each of the αS variants binds to SUVs with exchange kinetics that are slow on the NMR time scale, evidenced by the lack of significant broadening or shifting of any of the HSQC resonances, even while the intensities are attenuated due to lipid binding.
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Furthermore, the lack of CO2 line broadening demonstrates that during this exchange reaction, the CO2 is not released into solution but remains bound to the enzyme, as shown in eq 8. Surprisingly, the CO line broadening is independent of pH, remaining within the range between 560 and 1200 s−1 over the pD range from 5.5 to 9.0.
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It should be noted that the spin pair L141R1/I338R1 yields a mean interspin distance of 28.5 Å, which is in agreement with the model and consistent with the lack of any significant dipolar broadening in the corresponding CW spectrum seen in Figure 6.
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