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In fact, the researchers realized, the patients were acting a lot like a group of laboratory cats studied in the mid-1960's by a French sleep expert, Dr. Michel Jouvet.
To elucidate the pathogenesis of pandemic (H1N1) 2009 virus infection in cats, we studied 8 laboratory cats intratracheally infected with this virus.
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"We get the first clue as to their sinister leanings when they pick up a broom, grab Tabby the lovable laboratory cat, and, using the handle as a bat, fungo her out the window.
The DNA was further cleaned using PowerClean Pro DNA Clean-Up Kit (MO Bio Laboratories, Cat. No. 12997-50) and used for the library preparation for subsequent sequencing.
Specific IgM for TNP-OVA were measured by initially isolating the specific TNP-OVA IgM from plasma using Elisa plates coated with TNP-OVA, whereupon the bound IgM were measured using the rat IgM detection kit of Bethyl laboratories (cat nr.E110-100) astandardrd a pooled plasma was used of rats that were immunized with TNP-OVA.
Isoflurane (Abbott laboratories, cat. no. 571329.8).
D-Luciferin firefly luciferase substrate, potassium salt (Melford laboratories, cat. no. L1350).
The peroxidase substrate DAB kit (Vector laboratories cat# SK-4100) was used for colour development.
Secondary antibodies used for 3F4 and GFAP were a biotinylated horse anti-mouse IgG antibody (Vector laboratories cat# BA-2000) and a biotinylated goat anti-rabbit IgG antibody (Vector laboratories cat# BA-1000), respectively.
FITC donkey anti-mouse Fab'2 (Jackson ImmunoResearch Laboratories, West Grove, PA, Cat. No. 715-096-151) 10%00 inormalnormal donkey serum (Jackson ImmunoResearch Laboratories, Cat. No. 017-000-121) wandapplincubatedncubated for 30 min followed by two rinses in TBS.
Gram-positive and Gram-negative bacteria were cultured in Mueller Hinton broth (MHB) (Bacto laboratories, Cat. No. 211443) at 37 °C overnight.
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