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Field expression data was quantified relative to the signal intensity of control cDNA prepared from cauline leaves of plants grown in controlled environment chambers.
Therefore, the necessary intensity of control programs will vary considerably for different plantings and in different years, depending on individual orchard factors and weather conditions.
The averaged fluorescence intensity of control cells (white triangle) at 0 min was set as 100%.
The averaged fluorescence intensity of control cells (white triangle) was set as 100%.
Fold change for each gene was calculated by dividing the average intensity of 4 °C-treated samples by the average intensity of control samples at the corresponding time point.
For data analysis, quadrants were adjusted in raw data density plots of the fluorescence intensity of control samples in order to include a maximum of 5% of the cells in the upper-right quadrant of plots and then used in the analysis of the remaining samples to quantify the percentage of stained cells in the upper-left quadrant in comparison to agent free controls.
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The nature and intensity of controlling geochemical processes depend on soil type and fertilization.
This threshold was determined by the intensity of controls spots.
Syk knockdown was confirmed by FACS analysis: Mean intensity of controls was 409 (relative light units), for Syk knockdown 262 indicating a 60% reduction of Syk surface expression after subtraction of auto fluorescence (177).
Band intensity for proteins of interest was normalized to band intensity of controls (Flag for co-IP and β-actin for whole cell extract).
This approach models cases and controls as a marked heterogeneous Poisson point process with intensity λ(s) = λ1(s) + λ0 s), where λ1(s) is the intensity of cases and λ0(s) is the intensity of controls.
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