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Adherence was defined as a function of the instrument applied.
The approach is flexible, it is independent of chromatographic deconvolution method and analytical instrument applied.
Table 3 presents the specificity of the research instrument applied in the 31 studies that we reviewed.
Sequence reactions were purified by Sephadex G-25 (Amersham Biosciences, Otelfingen, Switzerland) before sequencing on an ABI 310 Genetic Analyzer (Perkin Elmer Instrument, Applied Biosystems, Rotkreuz, Switzerland).
Reactions were carried out on an ABI7900HT instrument (Applied Biosystems, Fosters City, CA) using standard procedures.
Cells were also examined on the Delta vision optical sectioning deconvolution instrument (Applied precision, Pittsburgh, PA) on an Olympus microscope.
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All PCR reactions were performed using an Applied Biosystems model 7000 instrument (Applied Biosystems, California, USA).
Quantitative reverse transcriptase PCR (RT-PCR) was performed using Taqman qPCR reagents and an ABI7500 fast system sequencing detection instrument (Applied Biosystems).
QPCR was performed in triplicate on an StepOnePlus instrument (Applied Biosystems, Thermo Fisher Scientific, USA) using the SYBR Premix Ex Taq II (TAKARA, Japan) reagent.
PCR was performed using ABI 7300HT and ABI Vii7 instrument (Applied Biosystems, USA).
Thermal cycling and data collection were performed using the ABI Prism 7900HT instrument (Applied Biosystems).
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