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In addition to the sex-specific transcripts, a set of common transcripts (fruCOM) is also produced from transcription that is initiated at alternative promoters, bypassing the target sequence for Tra and hence escaping sex-specific splicing [ 9, 10].
Subsequently, the target sequence acted as a primer to initiate the polymerization and nicking reactions, which released a great abundant of short sequences.
RNPs initiate DNA integration by recognizing DNA target sites, with most of the target sequence recognized by base pairing of the intron RNA and only a small number of bases recognized by the IEP [3] [7].
The target sequence was Bag3: 5′-GCCUGAAAACAAACCAGAATT-3′.
The target sequence is underlined.
The target sequences were as follows.
To activate these responses, Egr-1 must locate its target sequence and initiate the gene response within a short time, because of its limited lifetime in the nucleus (half-life of ∼0.5 1 h).
The Src siRNA target sequence was TGTTCGGAGGCTTCAACTCCT.
The control siRNA target sequence was AATTCTCCGAACGTGTCACGT.
The final target sequences were provided in the Supplementary Information.
The matching between the seed sequence (2 7 b.p). of the microRNA with the target mRNA sequence would suffice to initiate the regulation cascade by microRNA [ 38].
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