Exact(29)
Initial searching identified 773 citations.
Initial conditions: Suppose that the initial searching step L, which is the search length on the contour, is 1/5 of the character height.
The obtained sequences with 5 kb of flanking region were searched by using FastPCR v6.1 (26) to look for TIRs with initial searching word size of 12, filter minimal string length of 17, minimal alignment string length of 18, gap size between strings of 10, local similarity of 75 and alignment output length of 100.
Initial searching used TBlastN [ 36].
Initial searching of the Atlantic salmon genome database curated at the National Center for Biotechnology Information using the tblastn algorithm and zebrafish Aqp0a and -0b as input identified multiple aqp0-bearing contigs from which gene-specific primers were designed.
Initial searching of the Atlantic salmon genome identified 4 paralogous aqp0 genes, which, following molecular phylogenetic characterization (see below), were termed aqp0a1, -0a2, -0b1, and -0b2 (Fig. 1 ) The genomic sequences were used to design paralog-specific primers to amplify the full-length cDNAs from total RNA isolated from lens tissues, as well as the complete genes from genomic DNA.
Similar(31)
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The initial search focused mainly on Srebrenica.
An initial search in HOLLIS is recommended.
The company's initial search for land was unsuccessful.
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