Sentence examples for Initial polymerase from inspiring English sources

The qPCR cycling program was as follows; 15 min initial polymerase activation at 95°C followed by 40 cycles of 95°C for 15 sec, 58°C for 15 s and 72°C for 20 s.

The procedure used was initial polymerase activation for 30 s at 95 °C followed by 40 cycles of 95 °C for 10 s and 60 °C for 30 s.

PCR was then performed using equal amounts of DNA and the iTaqTM DNA ploymerase (Bio-Rad, Hercules, CA) under the following conditions: initial polymerase activation at 95°C for 5 minutes, 95°C for 45 sec, 53°C (methylated product) or 50°C (unmethylated product) for 45 sec followed by elongation at 72°C for 45 minutes.

After an initial polymerase activation and denaturation step at 95°C for 10 min, the samples ran 45 amplification cycles, each comprising denaturation (95°C for 10 s), annealing (60°C for 20 s), and extension (72°C for 10 s) in the LightCycler 2.0, with a temperature transition rate of 20°C/s for all steps.

PCR consisted of initial polymerase activation at 95°C for 3 min, followed by 36 cycles each of 30 sec at 95°C, 30 sec at 62°C and 5 min and 30 sec at 68°C, and a final extension step at 68°C for 5 min and 30 sec.

The AS-PCR procedure consisted of an initial polymerase activation at 94°C for 5 min, 25 cycles each of 30 sec at 95°C, 30 sec at 58°C and 5 min, and 40 sec at 72°C, and a final extension step at 72°C for 30 sec.

The reactions were incubated for 30 min at 50°C for cDNA synthesis followed by 2 min incubation at 95°C for initial Tag polymerase activation.

Spots might have better signal when the initial Taq polymerase amplification protocol used for all ORFs was optimal for their length, or when GIBCO Elongase was used on longer, reamplified genes.

PCR was conducted with the following program: an initial DNA polymerase activation at 95°C for 180 s, then followed by 40 cycles of 95°C for 30 s, 60°C for 30 s, and 72°C for 30 s. Finally, a melting curve was performed, and the PCR products were checked with 2% agarose gel in 1× TAE with ethidium bromide.

An initial Taq polymerase activation step of 4 min at 94°C, followed by a step of 35 cycles with 30 sec at 94°C, 1 min at 55°C and 1 min at 72°C, plus a final elongation step of 7 min at 72°C were performed.

Briefly, the thermal cycling conditions included an initial Taq polymerase activation (95°C for 3 min); the denaturation step (40 cycles at 95°C for 15 s); the annealing and extension steps (60°C for 1 min); and a melting curve analysis (incubation at 95°C for 10 s, then slowly decreased to 20°C).