Only in Craig Revel Horwood's The Indicator Line do we get a more blatant blast of testosterone.
On the other hand, and contrary to what had been observed with the Z/EG indicator line, recombination was widespread and could be observed in a number of tissues after treatment of the in DiCre×R26R animals with the inducer (Fig. 4).
Each identified spark was marked by a number and by a vertical indicator line transecting the peak of the spark.
To test for specificity of recombination using RIPCreER, the mT/mG indicator line was used that expresses membrane-bound tomato and GFP before and after Cre-dependent recombination, respectively (Muzumdar et al, 2007).
Second, with only a few exceptions, the expression level of fluorescent markers introduced by either knockin or BAC trangenics are often insufficient to label fine neuronal structures such as neuronal axons and synapses; expression levels from available indicator lines are orders of magnitude lower than what is necessary for high resolution imaging in vivo.
Using promotor indicator lines, where gfp expression is under transcriptional control of antimicrobial peptide gene promoters, no response could be observed using infection with Erwinia carotovora (data not shown).
Briefly, five 5-day soybean seedlings of each accession and seven indicator lines, PI 548402 (Peking), PI 88788, PI 90763, PI 437654, PI 209332, PI 89772, and PI 548316, were inoculated with 2,000 ± 25 eggs of HG Type 0, corresponding to race 3. A SCN susceptible cultivar, Hutcheson, was used as a check to evaluate the response to a nematode population.
Mitchum et al. [ 2] reported results of a survey showing that most of the SCN populations collected from Missouri soybean fields were virulent or could reproduce on indicator lines, like PI 88788, PI 209332, PI 548316, and Peking, used as resistance sources for soybean cultivars.
Continue turning the dial left until the indicator lines up with the second number of the combination.
When the indicator lines on the rulers reach the point(s) you want, release your left mouse button, and the object will snap to the ruler marks.
At that time, it would be useful to see how the technical indicators line up.
