Dentin Sialophosphoprotein (DSPP) positive matrix production was detected in the chamber, indicating functionality of dental pulp progenitor cells.
Fusion constructs and β-glucosidase alone displayed activity towards esculin and side-activity towards X-Gal, indicating functionality and the capability to be renaturated in SDS-PAGE.
As a result, the intracellular calcium concentration increased; hence, indicating functionality of voltage-dependent calcium channels which is essential for neurotransmitter release.
Predicted enhancers showed significant association with MTL indicating functionality and identified previously validated enhancers.
Considerable luciferase activity was detected when the claR upstream region was tested indicating functionality of at least one promoter embedded there.
Exposure of these individual cells to the promitogenic insulin analogue X10 that activates both the INSR and the IGF1R, resulted in intact downstream signaling cascades in all three cell lines, indicating functionality of the receptors (Fig. 1b).
Only a C-terminal mVenus fusion with a 9.4-kb genomic fragment containing intron, 5′UTR, and the upstream neighboring gene, provided full complementation of the mutant phenotype, indicating functionality of the protein fusion and of its regulatory sequences.
Immunostaining of polytene chromosomes from 43B-Gal4/UAS > Cap-H2-eGFP salivary glands using anti-Cap-H2 and anti-GFP antibodies revealed that Cap-H2-EGFP co-localizextensivelyely with endogenous Cap-H2, indicating functionality of the fusion protein.
Mbd3 −/− embryos express Cdx2 appropriately in early TE cells, demonstrating that Mbd3 is not required for Cdx2 expression in vivo (Kaji et al., 2007) (B.H., unpublished), are able to implant in vivo and to outgrow ex vivo, indicating functionality of early Mbd3 −/− TE (Hendrich et al., 2001; Kaji et al., 2007).
Expression per se does not indicate functionality.
Mere expression however does not necessarily indicate functionality.
