Exact(10)
The important difference in TLA between the geometry and topology experiments revealed that branching along the first annual shoot of the trunks enhanced tree architecture variability and variance in TLA.
A two-way, mixed-model design, analysis of covariance adjusted for baseline walking speed was performed to analyze changes in TLA and ankle moment across groups.
Postnuclear supernatants were subjected to a first ultracentrifugation (100,000 × g in TLA 120.2) to separate endomembranes from the cytosol.
The accessions with the greatest reduction in TLA in response to water deficit were Bur-0, Shahdara, Sakata, Mh-1, and Mt-0.
After an additional ultracentrifugation step (100,000 × g in TLA 120.2), the luminal fraction was harvested and supplemented with antibodies to Cnx, Crt, or to HA-Malectin to determine their presence.
The MDR- MTB clinical isolates were grown in TLA 7H11 and incubated for 3 weeks at 37°C in a 5% CO2 atmosphere until luxuriant growth became apparent.
Similar(50)
When compared with the two reference assays, MAT and IFAT, 5 false positive and 7 false negative samples were found in TLA-ELISA, 8 false positive and 10 false negative samples were found in GRA1-ELISA, and 5 false positive and 5 false negative samples were found in GRA7-ELISA (Table 1), demonstrating that the GRA7-ELISA showed the highest co-positivity and co-negativity rates (Table 3).
Polysomes from Giardia trophozoites were prepared [70] by gently lysing the cell by dounce homogenizer and pelleted at 78,000xg for 4 hours in a TLA 100.3 rotor (Beckman), and resuspended in buffer A (20 mM Tris-Cl, pH 7.5, 4 mM MgCl2, 50 mM KCl, 2 mM DTT, complete protease inhibitor) to a final concetration of 100 OD260/mL.
To separate inner and outer membranes, ∼320 μl of membrane samples were laid on top of 3 ml 18% Percoll (vol/vol in buffer A), followed by ultracentrifugation at 30,000 rpm in a TLA 100.3 rotor at 4°C for 15 min (Morein et al., 1994).
The supernatant medium was centrifuged at 20,000× g for 30 min and the resulting supernatant was centrifuged at 200,000× g for 60 min in a TLA 100.3 rotor (Beckman) to sediment membrane vesicles, which were resuspended in N-free medium or HMDEK (20 mM pH = 7.2 HEPES, 5 mM MgCl2, 1 mM dithiothreitol, 1 mM EDTA, 25 mM KCl) buffer.
Newly formed proteoliposomes were subsequently purified by floatation using ultracentrifugation for 210 min through a sucrose gradient (0 25 30% of sucrose) in a TLA 120.2 rotor at 100,000 rpm.
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