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The CIE-induced and demethylation agent, 5AZA-induced increases in transcription factors occupancy in this sites within the NR2B promoter were also confirmed by ChIP Assay (Figure 8).
In Drosophila, POLQ acts in this sites where its helicase activity unwinds short stretches of DSBs to expose pre-existing microhomologous sequences that are used to align the broken ends to provide a template for pol θ polymerase activity [ 19].
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