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Annexin V-staining of adherent cells revealed only a few apoptotic cells in this differentiated U937 cell fraction.
This is a very unusual organization of the Golgi apparatus in a post-mitotic mammalian cell and is likely to reflect the spatial features of the endomembrane system in this differentiated cell type.
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Interestingly, this differentiated scoring in addition to tree labeling resulted in reconstruction of "parsimonious" evolutionary scenario.
In this study, differentiated neurons could still adhere to the EVAL GYIGSR surface with functional synaptic activity after incubation for 20 days.
In this study, differentiated ESCs were shown to have fibril networks, resulting in compact cell aggregates (Fig. 1B, C).
Therefore, in this neuronally differentiated cellular setting, A53T seemed to cause lysosomal damage that was more generalized and not confined to CMA.
It is likely that COPI-mediated intracellular transport may be abundant in this highly differentiated secretory tissue compared with other tissues.
In this system, differentiated suprabasal keratinocytes presumably avoid apoptosis due to the permissive environment of the epidermis, as they readily undergo apoptosis when removed from this environment [ 8].
In this method, differentiated cells were first loaded with 150 nM MitoTracker Green FM for 30 min at 37°C and then fixed and imaged as described above.
The introduction of metronomic capecitabine could account for the high response rate obtained in this well-differentiated subset.
Using our anti-TAp63 antibodies, so far we have failed to detect TAp63 in the differentiated mouse keratinocytes — this could be due to species-specific or age related differences.
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CEO of Professional Science Editing for Scientists @ prosciediting.com