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In these arrays PRKD1 gene expression was drastically reduced in most cases analysed [ 41- 44].
In these arrays, each channel, with 48 wells, contains pre-designed primer probe sets for 12 different genes (in quadruplicate).
In these arrays, a print-tip is used three times to print a block in each metablock.
In these arrays, wtLNCaP and LNCaP-BIC cells with knocked down PRDX-3 (showed3) showed upregulation of a number of pro-apoptotic markers including p21, Fas ligand and cleaved caspase-3, but not Bad.
In these arrays the probe sequences come in pairs: for each perfect match (PM) probe, which is exactly complementary to the transcript sequence in solution, there is a second probe with a single non-complementary nucleotide with respect to the specific target.
In these arrays, a majority of the genes were derived from duplications occurring after the GA split but were shared between spruce and pine, indicating from the perspective of geological time that expression divergence may occur quite rapidly after gene duplication [ 51].
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Our initial aim was simply to mimic the situation in which these arrays would be used in an actual genome-wide study in any of the populations represented by the HapMap 3 collection.
There were more than 2.8% (216/7597) distinct genes either upregulated (ratio>2) or downregulated (ratio<0.5) in these array experiments.
Identifying possible families in these array patterns may be important in understanding the evolution and spread of pathogens such as M. tuberculosis.
The dispersal to multiple chromosomes may be related to the large segmental duplications in which these arrays are embedded.
Besides, even in sparse cultures, these arrays cannot provide information about the sub-cellular locus in which the electric activity is being generated.
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