The secondary step differed in extension time (72°C for 1 min).
Another scenario is, however, possible, which is to a certain degree related to the Dobzhansky-Muller model in that it would involve multiple steps, but those would occur in sequence, and not independently: the secondary steps of isolation would target traits specific to the saeptated population which could quite possibly be the one having driven the saeptation, but not necessarily.
In the secondary screening step, the isolated helper strains were inoculated into fermentation medium (15% l-sorbose) and cultivated in 250 mL flasks at 29 °C and 180 rpm for 12 h.
In the secondary antibody step, the membrane was soaked in 50 ml of 1∶5000 diluted goat anti-mouse IgG/M HRP (or using 1∶4000 goat anti-rabbit IgG HRP if the primary antibody was from rabbit) in Wash buffer for 50 minutes, followed by 5 washes for 5 minutes with 50 ml Wash buffer.
In a secondary step, EHMT2 is recruited to the Slc22a2 and Slc22a3 promoters and is required to maintain repression of these genes [ 35 ].
DNA methylation, in contrast, is a secondary step in the silencing pathway under study.
For DAPI staining, DAPI was included in the secondary antibody incubation step at a dilution of 1 2000.
For example, Qdot labeling was quite poor when serum was used as the blocking reagent in the secondary antibody labeling step, rather than albumin (data not shown).
18 Carbon dioxide is the major gas produced during roasting (87% of gases released from roasted coffee together with 7.3% of carbon monoxide), formed as a result of the many reactions occurring, among which Strecker degradation: a secondary step in the Maillard reactions and pyrolysis of carbohydrates.
Therefore, a secondary step in the analysis may be necessary to "unpack" the compiled score and parse out differences in community types.
