Sentence examples for In the one step from inspiring English sources

The detection limit was estimated to be 1 fg, corresponding approximately to one organism, while in the one step PCR previously described 4×102 organisms were required.

The update of the observational probabilities is done the same way as in the one step case, taking into account which variables were intervened.

These included one gap on G2, G3, G5, G7 and G8, and three gaps on G1 in the One-step map, and one gap on G2, G3, and G7, two gaps on G5, and three gaps on G1 and G8 in the Two-step map.

Significance at the patient level is estimated based on p1 and p2 in the two-part models and based on p2 in the one-step models.

As summarized in Figure 4, methods consisted in the one-step, total IgG-IgA fractioning on affinity sepharose columns; the two-step, IgG and IgA separation on sequential columns; the three-step, IgG affinity separation, followed by IgA isolation by ionic exchange plus gel filtration; the IgA1 affinity separation on a jacalin-agarose column.

Aniline was polymerized in the presence of HPA in the one-step preparation method, while HPA was immobilized on the ready-made PANI support in the two-step method.

Duplex RT-PCR was performed on an iCycler real-time PCR system Bio-Rad Laboratoriess, Hercules, CA) in the One-step TaqMan® RT-PCR Master Mix Reagents (Roche, Branchburg, New Jersey) according to the manufacture's instruction with target gene primers, target probe, housekeeping gene primers and housekeeping gene probe in the same reaction [46].

Duplex RT-PCR was performed on an iCycler real-time PCR system Bio-Rad Laboratoriess, Hercules, CA) in the One-step TaqMan® RT-PCR Master Mix Reagents (Roche, Branchburg, New Jersey) according to the manufacture's instruction with target gene primers and probe and internal standard gene primers and probe in the same reaction.

In the one-step method, the temperature was increased directly to the pyrolysis temperature.

Micropores were not formed for the carbon films heat-treated at 700 °C in the one-step method.

In this study, we prepare GaAs NWs by a facile two-source method in the one-step growth platform.