Sentence examples for In the first strand from inspiring English sources

The primers used in the first strand synthesis and the PCR amplification are listed in Additional file 1: Table S2.

The primers used in the first strand synthesis were cDNA 3′ SMART CDS Primer II A, 5′-AAGCAGTGGTATCAACGCAGAGTACT (30) VN-3′ (N = A, C, G or T; V = A, G or C), and SMART II A oligonucleotide, 5′-A AGCAGTGGTATCAACGCAGAGTACGCGGG-3′.

We assumed that the precise sequence of the T7 primer is important in this respect because the complete T7-primer is incorporated in the first strand cDNA generated by an RT enzyme upon annealing to the poly-A tail of mRNA extracted from samples.

Furthermore, we found by substituting T7-Oligo dT primers for T7N6 in the first strand synthesis in the ANRO process that about 20% of the biotinylated NRO material was already polyadenylated (data not shown), indicating rapid processing of primary transcripts to mature mRNAs.

cDNA synthesis was initiated using an oligo (dT) primer, using methylated C in the first strand synthesis reaction.

Briefly, total RNA (5 μg) was reverse transcribed using T7-Oligo (dT) primer in the first strand cDNA synthesis.

In latent serpins, the first strand of the C-sheet has to peel off to allow full RCL insertion.

Then place the last one in the loop of the first strand.

The first strand in Albanese's rope was the discovery of midazolam in Thomas's system.

Minus RT control reactions were done by synthesizing the first strand in the absence of StrataScript enzyme.

The oligo dT 20 primer was used in reverse transcription to synthesize the first strand cDNA.