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Other than in Newtonian and Laplacian physics, we cannot determine and predict motion or direction in the first dimensions of this model.
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In the first dimension, the amino acid enantiomers are separated as their d plus l mixtures by the reversed-phase mode, then the d-amino acids and their l-counterparts are separately determined in the second dimension by the enantioselective columns.
The system consisted of an ion-exchange column in the first dimension and the twelve reverse-phase columns in the second dimension; all thirteen columns were monolithic and prepared inside 250 µm i.d.
The purified racemate from the achiral chromatography in the first dimension is then transferred to the chiral column in the second dimension to conduct the enantiomeric separation and analysis.
The average run-to-run reproducibility of 43 components for six replicate injections was found to be 0.12% RSD in the first dimension, and 0.74% RSD in the second dimension.
This technique separated proteins according to their isoelectric point in the first dimension and according to their molecular weight in the second dimension by SDS-PAGE.
By neutral loss scanning, the cross peaks of a given ion in the first dimension with the building blocks present in the second dimension represent the fatty acyl chains of each triglyceride.
Figure 5 Cell culture supernatant proteins of Mycobacterium tuberculosis separated by a narrow range pH gradient between pI 4 and 5 in the first dimension and SDS-PAGE in the second dimension.
All items had positive factor loadings between.46 and.74 in the first dimension, and between.38 and.77 on the second one.
The t-values for the weighted mean squares in the first dimension indicate that one of the constrained items discriminated too strongly in the first dimension and that two others did not discriminate sufficiently (t = 2.6).
For 2-dimensional gels, proteins were separated using a pI range 4 7 strip in the first dimension and a 12% SDS-PAGE homogeneous gel in the second dimension.
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