In the first dimension, the extract was eluted with a gradient of water/methanol over a cross-linked dextran gel Sephadex LH-20 column.
In the first dimension, 4 μL of sample was loaded onto the polysulfoethyl, a strong cation-exchange (SCX) column (0.32 × 50 mm, 5 μm).
In the first dimension, agents exercise visible power when they directly influence political decision-makers based on their material and ideational resources [42].
In the first dimension, the three subpopulations as defined in STRUCTURE form three clusters with overlap.
In the first dimension, 2.0 µM protein was resolved according to its isoelectric point (pI) by isoelectric focusing (IEF) using Biorad ReadyStrip™ 7 cm IPG strips, pH 3 10 range at 800 V for 17 h.
In the first dimension, 150 µg of protein samples were run on 24 cm Immobiline DryStrips (GE Healthcare) of pH range 4.0 7.0 on an IPGphorII IEF system (GE Healthcare) as recommended by the manufacturer.
In the first dimension, pH 4 to 8 ampholytes were used.
In the first dimension, protein molecules are resolved depending on their isoelectric point (pI) [ 2].
In the first dimension, phosphopeptides were injected onto a sulfated-ion exchange column (PolyLC 0.3 mm × 50 mm (Columbia, MD).
In the first dimension, the same column was kept throughout the experiments (DB-5 column, non-polar).
In the first dimension, the extended D-loop was separated from D-loop anD1D1 oligonucleotide under native conditions.
