The EcoRI and BamHI sites were eliminated sequentially by digestion, filling in the ends using Klenow enzyme, and religation which resulted in the intermediate vector pPZP650.3 (not shown).
After filling in the ends, two rounds of PCR amplification were performed to enrich for the desired cDNAs containing both adaptors.
In "The Ends" he described the torture and suffering he endured as a political prisoner.
"Growing up in the ends, you don't do that.
So Melanie's hair has a gentle curl in the ends.
He stopped and was like: 'Yo, you're back in the ends?
Fold in the ends of the parchment, then pull it up around the fish.
He will ride with a couple of big, impressive pumpkins in the ends of his saddlebags.
Fold the dough over the filling then roll tightly, tucking in the ends and sealing the edges with water.
I'm cool and I'm a good guy but in terms of being interesting… I felt like I'd be in the ends all my life.
I think it's up to black actors and writers to create new parts that are not always set in "the ends".
