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In the differentiated, pOPTN-EGFP-transfeced RGC5 cells, punctate optineurin-GFP foci were found on the neurite extensions (Fig. 3B).
In the differentiated histology group, BMP-7 expression was not associated with postoperative outcome.
In the differentiated LAMA-84 cells, we observed accumulation of proapoptotic tLivin concomitant with increased caspase-3 activity.
In addition, approximately half of the differential miRNAs do not target any genes in the differentiated gene list.
Cd44 at the protein level also decreased with differentiation, although occasional cells remained positive also in the differentiated stage.
Keratin 76 (Krt76) is expressed in the differentiated epithelial layers of skin, oral cavity and squamous stomach.
GAG and total collagen content in the differentiated cells increased significantly with in vitro culture time.
Surprisingly, PTEN expression significantly decreased after TNFα treatment in the differentiated C2C12 cells, suggesting it was not likely responsible for Akt phosphorylation downregulation.
In addition, Alcian blue staining was used to detect the formation of sulfated glycosaminoglycans (GAGs) in the differentiated cells.
At various stages in development unequal segregation of molecular information must occur to result in the differentiated characteristics which distinguish cell progeny.
We turned our attention to cathepsin B which is known to be abnormally synthesized in de-differentiated chondrocytes and scarcely produced in the differentiated ones.
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