Exact(60)
In the assays we did not find cross reactivity.
In the assays excretions/secretions and secretions alone were tested simultaneously.
In the assays shown in Figure 7A,B, the concentration of plexin was 2 µM.
The solutions were stored at −20 °C before they were used in the assays.
The MH medium was first used in the assays, according to the CLSI recommendations.
Compounds were diluted ten-fold in 1% (v/v) DMSO and used in the assays.
Moreover 6v is devoid of any inhibitory activity in the assays to other thirteen protein kinases.
In the case of the 68Ga-labelled compounds, the final peptide concentration in the assays was 0.2 nM.
Negative controls were included in the assays wherever required.
Receptor activator of NFκβ (RANKL) was included in the assays as a positive control of osteoclastogenesis.
Cryopreserved cells were stored in liquid nitrogen until used in the assays.
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