In the P1 passage we observed only 2.1±1.2 colonies per mouse, while in the P3 passage we observed an almost 20-fold increase in lung colonization (37.9±18.7 colonies per mouse; Figures 1 and 3).
Finally, to provide mechanistic evidence of the dependence of STAT1 on the observed aggressive STAT1H genotype, we generated stable STAT1 knockdowns in the P2M5A cell line, which was the highest overexpressor of STAT1H in the P2 passage (Figure 3).
The same pattern is evident in the 70-passage set, where the first sample exhibits moderate up-regulation while the other three exhibit moderate down-regulation.
This field strain was gradually attenuated by 840 continuous passages in TSB medium, and the 840th passage was named strain YM001 [ 18].
qRT-PCR was performed with various endodermal markers and data analyzed for changes in marker expression over the 7 passages in serum containing and serum-free conditions.
We detected fixation of mutations in the consensus sequences only in 7 out of the 12 passaged viruses.
However, mild down-regulation is consistent in all four samples of the 50-passage group.
Of the 58 private apartments in the 1 Lambs Passage development, 71% had no voter registered, while 65% of the 106 in Worcester Point had no one registered.
The growth of carcinoma of the human large bowel was studied in the first 2 passages in immune deprived mice.
This observation suggested successful engraftment of the scaffolds to bone in the 3 mm passages.
