Exact(5)
IN catalyzes the first step of the integration process, namely the 3'-end processing in which IN removes a pGT dinucleotide from the 3' end of each viral long terminal repeat (LTR).
Products are analyzed by polyacrylamide gel elctrophoresis (PAGE), and are evidenced by a decrease in size of the substrate after IN removes two deoxyribonucleotides from the 3' end.
IN removes two adjacent nucleotides, guanine (G) and tyrosine (T), from the 3′ end of U5 or U3, the so-called 3′-end processing (3′P).
14– 16 HIV-1 IN removes the terminal dinucleotide (GT) adjacent to invariant CA nucleotides from each 3′-end of blunt-ended viral DNA, referred to as the cleaved strand.
Plasma exchange is the most important treatment, as it replaces large volume of plasma containing the missing enzyme and in removes the associated anti‐ADAMTS13 IgG antibody, which is directly related to the pathogenesis of the disease.
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