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In polymerase chain reaction (PCR) RNase activity is removed by adding inhibitors.
In polymerase β it has been suggested that triphosphate-sugar binding occurs before there is a check for complementarity in nucleotide insertion [31].
In polymerase structures from all groups, the catalytic palm domain incorporated a ferredoxin-like fold (fig 3a, green) that was variably decorated by a number of additional domains.
In polymerase chain reaction (PCR -Southern blot analysis, HPCR -Southerndetected from 22 out of 42 SCCs (52%) with predominance of HPV-16 (68%).
In polymerase chain reaction (PCR), 50 ng of genomic DNA was used in 30 μl volume of standard DYNAZyme II (Finnzymes, Finland) PCR reaction mix.
DNA amplification was accomplished using Phi-29 polymerase (Lucigen-30221) in polymerase buffer using the same conditions overnight.
Using this tool, we demonstrate spatial heterogeneity in Polymerase II dynamics in live U2OS cells.
Specific primers can be used in polymerase chain reactions (PCR) to amplify prey DNA from the gut content of generalist predators at high specificity and sensitivity.
Seventy two primer sets in the flanking region of dinucleotide, trinucleotide and tetranucleotides repeat motifs were designed and tested for efficiency in polymerase chain reaction amplification.
However, recent advances in polymerase engineering and templated nucleic acid synthesis have enabled the direct selection of aptamers having modified backbones and nucleobases.
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Nicks or gaps between the adaptors and the dscDNA fragments were repaired using the fill-in polymerase.
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