Sentence examples for In plb from inspiring English sources

A reduction in PLB size leads to reduced total Pchlide levels (Franck et al. 2000; Sakuraba et al. 2013).

When the kinetics of Ca2 + pumping into the SR were increased by an increase in PLB phosphorylation (via PDE and PP inhibition or addition of cAMP) or by 2D12, self-organized, "clock-like" local Ca2 + releases, partially synchronized in space and time (Ca2 + wavelets), emerged, and the ensemble of these rhythmic local Ca2 + wavelets generated a periodic high-amplitude Ca2 + signal.

With a decrease in SERCA2a expression and an increase in PLB expression, the SERCA2a/PLB ratio is significantly decreased, leading to a slower relaxation.

The samples were split into 2×1 ml fractions and precleared with 50% protein A-agarose (Sigma) in PLB by incubation rotating at 4°C for 1 h.

The protein A-agarose beads were resuspended in PLB containing 0.1% SDS and 30 µg proteinase K (Sigma) and incubated at 50°C for 30 min. An equal volume of phenol∶chloroform∶isoamyl alcohol (24∶24∶1) was added to the protein A-agarose beads and the aqueous layer containing the RNA removed.

Similar inhibition was produced by an artificially evoked burst of spikes in PlB, indicating that increased firing in PlB was sufficient to suppress sucrose-induced feeding.

However, while the compensations in PLB-KO myocytes are minute and the phenotype is mainly due to ablation of PLB, the CaMK-TG phenotype seems to be induced mostly by secondary changes in gene expression.

In order to monitor the generation of membrane potentials we performed a FLIPR assay on the CcO embedded in PLBs using the FRET pair CC2-DMPE/DiSBAC2(3).

Using qPCR and a self made antibody, we detected a significant amount of Hv1 in human eosinophil and neutrophil granulocytes and in PLB-985 leukemia cells.

Similar data on SBDS mRNA downregulation over time were observed in the original HL-60 myeloid cell line differentiated into neutrophil-like cells (n = 3; data not shown), confirming the data in PLB-985, which represents a subclone of the HL-60 cell line.

As expected, the increased systolic Ca2+ concentration in PLB-KO causes a substantial rise in the activity of CaMK and CaN.