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However this explanation does not seem to hold since the GC% of the 18S rRNA gene in our sorted populations is only marginally higher for Haptophyta compared to the other groups (Table 3).
The expression of arterial, venular and lymphatic markers was detected in freshly FACS-sorted samples and maintained in culture up to 70 days, indicating that all three types of endothelial cells (arterial, venular and lymphatic) are included in our sorted starting population and their phenotype is maintained in culture.
We were unable to test the presence of CACs in our sorted CD133+ fraction due to a lack of specific "human" CAC marker.
Comparison of the transcriptomes revealed that 3,419 genes were detected in our sorted cell transcriptomes but not in the whole tissue (see Additional file 2).
For each previous study, we then noted where the reported differentially expressed genes were located in our sorted list, and assessed the resulting significance using a Z score to measure divergence from a random distribution.
As immune cell infiltrate is a large component of stromal tissue, we decided to use antibodies to allow us to quantify and negatively select immune cells to avoid contamination in our sorted epithelial fraction.
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The moderate sizes of Pareto fronts in our applications also imply that no benefit is to be expected from using quad-tree data structures in place of our sorted list representation.
Knowing that we might miss genes that are expressed in subsets of our sorted neuronal subpopulations, we assumed that, below 1,000 number of reads, transcripts' detection by ISH would be difficult to monitor.
The positions in our concatenated alignments were sorted based on their character state variation.
In our microarray data for sorted MEC subsets, as well as those publicly available microarray datasets we analyzed, we could always observe a largely opposite expression pattern of Elf5 and Runx1.
Hence, the process can be continued in the same fashion, outputting the hyperpaths in H in sorted order.
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