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Our identification and annotation of all experimentally supported transcript models leads to an enrichment of AS transcript present in our geneset compared to those found in other manually curated genesets.
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However, creating large curated data products, such as those commonly used in genomics (e.g., [ 29, 30]), or through networked observatories, requires methods that are done 'at scale', in other words not manually, and that are automated and extensively documented.
Sequences were also compared to the pathogenicity genes assembled in the PHI database [ 2, 44] and to the genes linked to the mating-type in other fungi using a manually built list of such genes.
As the measurement technique, the BP recorder, in our study was identical in the two settings, our results of similar BP, whether measured by SMOPB or OBP, suggest that the higher BP recorded manually in other studies could to some extent be dependent on the lack of expertise.
The Florida Supreme Court has made another with its order today that thousands of ballots in Miami-Dade County be manually recounted, along with thousands in other counties where no manual recount has taken place.
The responses of some of the big CSPs (e.g. Amazon, HP, Microsoft, RedHat, or SAP1) who provided answers in other forms, though, were processed manually to ensure the consideration of the major players.
The electron density cutoffs were obtained from the investigators in most cases; in other cases the cutoffs were manually approximated to reproduce the published EM objects.
We extracted SBP values by means of a self-written text mining algorithm based on regular expressions, a method which has proved valuable for extraction of numerical variables in other settings [ 9] and manually validated in 500 files for this cohort with a sensitivity of 95.8%95%5% confidence interval (CI) (91.2, 98.5)) and a specificity of 100%9595% CI (99.0, 100)).
Nevertheless, there are cases where software is installed in other ways, either by compiling it manually or by installing software from binary packages that are not available in repositories.
However, it is important to mention that in the case of determining the gene structure of pfsr12 orthologues in other Plasmodium species we had to manually inspect and correct the predictions of introns/exons.
We used the Chalkley counting method for vascular quantification which measures the relative vascular area [ 45], as recommended in a consensus meeting for quantification of vascularity in solid tumors [ 39], whereas in other studies microvessels have been counted manually [ 15, 19, 20] or using image analyses [ 6].
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