To examine whether origin activity was conferred to NUMTs by the ACS motif, we constructed plasmids carrying ARS and NUMTs (in both orientations) where the 17-bp ACS motif was replaced with a G+C rich sequence.
From the conformation with the lowest CDOCKER interaction energy structure of M100G scFv anti-p17, the peptides bound in two orientations, where the N-terminal (p17.1 p17.6) and the C-terminal (p17.7 p17.9) of peptide sequences were directed toward the binding pocket.
For paired-end data, it ensures that reads are in both the orientations where SNVs are called.
Rouboa et al. [ 11] simulated a hand/forearm model with thumb adducted in three different orientations where water flow velocity was increased from 0.5 m ·s−1 to 4 m ·s−1.
Both organelles are mostly oriented parallel to the longitudinal axis of the tube in the shank, but exhibit different orientations where the cytoplasmic streaming changes direction.
Taurine hydroxylase (TauD) also shows a weak water ligand in complex with α-KG [22] and the crystal structure displays the carboxylate moiety in an orientation where the non-coordinating oxygen of the carboxylate ligand is flipped down and away from the open (i.e. water) coordination site and will therefore not partake in the stabilization of the water ligand [25,26].
Throughout the shared SDR, there are no obvious genes that sit in an orientation where a terminator sequence would be recognized short of transcribing RNA-directed DNA polymerase from mobile element jockey-like, which is consistent with our proposed model of transposition.
In a second scenario, the epoxide could bind in the active site of Cg10062 in a different orientation where the carboxylate group interacts with Arg-70 and Arg-73.
For this construct, when the dsDNA binds to the filament in the correct orientation, where the unlabeled homologous end is in close proximity to the acceptor, FRET stays low and these events were not included in the histogram shown in Figure 4 Figure Supplement 4B.
We found that with P4MP1 layer thickness smaller than 400 nm, a confinement effect will induce in-plane P4MP1 crystal orientation where the crystal c-axis is perpendicular to the film.
