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This resulted in good expression levels of soluble protein of most of these genes.
Co-transfections in A2780 cells resulted in good expression of p21 and PCNA, as seen in immunoblots of cell lysates.
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In other words, a good expression level is one of the characteristics of a stable receptor.
Additionally, the half DVD-Ig molecule has good expression in mammalian cells, can be purified to homogeneity, and has improved half-life compared to other monovalent antibody variants (e.g., scFv, BiTE, and Fab).
Since our earlier work with Trichoderma virens genes have shown good expression in plants [23], we chose a unique GST gene of Trichoderma virens for incorporation into tobacco for developing Cd tolerance.
The ChAT Cre driver has previously been shown to give good expression in motor neurons.
With these constructs, 11 BVMOs could be obtained in soluble form with good expression levels, using E. coli TOP10 as host (Table 1).
In order to obtain good expression of tyrosinase, the plasmid pMFvpt was always transformed freshly into E. coli JM109 cells during the entire study.
Although Volvox diverged from unicellular ancestors like Chlamydomonas at least 200 million years ago and codon usage became different from each other [ 43], the relationship obviously is still close enough to allow for good expression in Volvox.
We chose the promoter of the Vav gene, expressed in the entire hematopoietic lineage but few other cell types, showing good expression levels in all cell types of the blood, including multipotent progenitors as well as haematopoietic stem cells [6].
It was desirable that the expression data of a gene exhibit good expression dynamics in at least one of the datasets.
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