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The potential GLUT transportability of the complexes was investigated using cell-based fluorescent competition assay and GLUT inhibitor mediated cell viability analysis in GLUT over-expressing HT29 cell line.
The neomycin group showed greater GAG retention after both 10 and 50 million accelerated fatigue cycles and after 1 year of storage in GLUT solution.
The pseudo first order Lagergren [42] model presumes that the rate of variation of solute uptake by reaction time is certainly related to versatility in glut concentration and solid uptake value via reaction time (14).
Since Akt has a role in GLUT vesicle trafficking, the role of Akt in PIA and Per-induced vesicle release was assessed.
In humans, 23, 38, 2, 1 and 41 mutations have been reported in GLUT 1, GLUT 2, GLUT 4, GLUT 10, and the SGLT 1, respectively.
Since β- Actin transcript levels can change in heart failure [ 19], the relative changes in GLUT expression in murine models of cardiac hypertrophy compared to controls animals were determined using the ΔΔCt method with HPRT serving as an internal control.
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Increases in GLUT-1 levels in vemurafenib-treated A375R1s were apparent using a more sensitive immunofluorescent histological approach [Additional file 6: Figure S6B].
The exception to this were the HS294T and RPMI-7951 melanomas, in which FDG response was induced by cell death rather than glucose metabolism; this was supported by the observation that vemurafenib and GDC-0973 were unable to induce changes in GLUT-1 at the membrane [Additional file 2: Figure S2]. Figure 1 BRAF and MEK inhibition of FDG uptake.
We did not detect changes in Glut-4 mRNA levels with estrogen in any muscle or time point tested.
In adipocytes and muscle cells, Sx 4 was shown to participate in GLUT-4 exocytosis [26], [27].
These data are consistent with the biochemical studies, suggesting an inhibitory role for ATP in GLUT-mediated glucose transport.
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