Exact(10)
In first passage cells, the expression of transcription factors regulating collagen, including SOX-5, -6, and -9, appeared to be even higher in OA chondrocytes.
Critical excitations play an important role in first passage problems because they are the most probable point in the first passage failure region of the standard Normal stochastic load space.
Processes involving cell proliferation are shown to be "nonlinear" (even though mean-field equations for the expected particle numbers are linear) resulting in first passage time statistics that depend on the position of the bottleneck or oasis.
To extend the IHC results, cells were isolated and analyzed in first passage by flow cytometry.
For generation of MMO in first passage, MCF7 remained in non-adherent conditions for 10 days.
Signaling was measured in first passage subcultures by treatment with cytokines and growth factors for 15 min followed by immunodetection of the phosphorylated signaling proteins (representative example in Fig. 3).
Similar(50)
In third passage, the cells were grown up to 80 90% confluence in 25 cm2 tissue culture flasks (Corning).
To exclude inflammatory and hematopoietic cells, adherent cells were passaged three times, and osteoblastogenesis again induced in fourth passage (P4).
This effect was dependent on co-stimulation of the EP4 receptor; however, in rat, the EP4 receptor was not detected, at least in fourth passage chondrocytes [ 46].
Two aliquots of 1 2 million MSCs per culture, from 3 BM and 3 PL samples in third passage, were lysed and frozen in TRIzol reagent (Invitrogen).
In second passage of NBH-annealed fibrils, the two groups of animals inoculated with BHs from #36793 and #36798 showed different incubation times to onset of disease.
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