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In FACS results, this system showed excellent gene transfection efficiency depending on irradiation power.
In FACS assays, 3.8% of CD4+ cells produced IFN-γ when stimulated by the extract.
In FACS studies, intracellular oxidation of C-400 was measured in the FL1 channel.
After treatment, the cells were harvested in FACS flow (BD Science) and analyzed by FACS.
For analysis, the cell pellets were resuspended in FACS buffer.
All mutants maintained effective antigen binding in FACS analysis and BIAcore (Additional file 1: Table S1).
After incubation at +37 °C in the dark for 30 min, cells were washed twice in FACS buffer and fixed with 4%% paraformaldehyde at room temperature for 15 min, then resuspended in FACS buffer and analyzed by flow cytometry.
Cells were then washed twice in FACS buffer.
The cells were finally washed and analyzed in FACS Calibur.
We thank Ruth Didier for her assistance in FACS analysis.
Cells (106 per tube) were pelleted in FACS buffer (PBS, 3% BSA) and were re-suspended in liposomal preparations diluted 1/10 in FACS buffer.
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