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The fixed cells were dehydrated in ascending concentrations of ethanol (70%, 80 %, 90 %, 95 and 100%) and embedded in EMbed 8100%esins (EMS, Wandington, PA, USA) via propylembeddede.
The cells were embedded in EmBed 812 DER 736.
The pellet was stained en bloc with 2% aqueous uranyl acetate followed by ethanol dehydration and embedded in EMbed (EMS).
The samples were then embedded in EMbed.
Tissue was embedded in EMbed 812 and polymerized at 60 °C for 1 2 days.
After dehydration in a graded series of acetone, the cells were embedded in Embed 812 epoxy resin.
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Watermark extraction follows the basic procedure used in embedding.
This is a well-known concept in embedded systems design.
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