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In CEP, drug doses were epirubicin 80 mg m−2, paclitaxel 175 mg m−2 and cisplatin 75 mg m−2.
The CHO-to-protein ratio in CEP was 2 1.
Interestingly, despite a clear increase in CEP levels in s.c. models, no significant differences in CEP levels were observed at any time point with any cell line in the orthotopic xenografts.
FeNO may be a useful marker for monitoring eosinophilic parenchymal inflammation and determining the appropriate corticosteroid dose in CEP.
FeNO may be useful for monitoring eosinophilic parenchymal inflammation and determining the appropriate corticosteroid dose in CEP.
This mobilization effect in CEP levels may facilitate tumor cell repopulation during the common time intervals between the individual chemotherapy cycles [ 33].
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To a 96-well ELISA plate was added 100 μL of 5 μM CEP in CEP-HSA protein in PBS solution into each well, and the plate was incubated at 37 °C for 1 h.
These results indicate that numerous ncRNAs are involved in CEP-1 transcriptional network and that these are especially enriched on the X chromosome in C. elegans.
Our data showed that in cep-1 mutant (gk138) worms TTS-1 and TTS-2 were up-regulated 2-fold and 7-fold higher respectively in wild-type worms under normal growth conditions (Fig. 7B).
Our result showed that not only is the expression of ZK355.8 up-regulated more than 8 fold after UV irradiation in wild-type worms (N2/UV), but also that the expression of ZK355.8 in cep-1 mutant (gk138) worms was about 4-fold higher than in wild-type worms, and increased 2-fold in cep-1 mutant (gk138) relative to wild-type worms N2 after UV irradiation (Fig. 7A).
We also validated the differential expression of one component of the snoRNP complex, the GAR-1 gene, which is upregulated in cep-1 mutant (gk138) worms under normal growth conditions (Fig. S2) and contains the conserved CEP-1 binding motif in its promoter region (AAACTTGCCC, located in 2796 bp upstream of TSS).
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