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In CEF, v-Src transformation stimulated the expression of four critical regulators of actin polymerization and podosome formation, namely N-WASP, cortactin, gelsolin and the p41 subunit of the actin related protein complex 2/3 (Arp 2/3 subunit 1b-p41; Additional File 3[ 81- 84]).
Finally, we find strong evidence of decreasing returns to scale in CEF management.
The attenuated Gt strain, however, is not virulent in chickens and replicates well in CEF cultures.
Results showed that recombinant viruses were generated and correctly expressed foreign genes in CEF, BHK-21 and 293T cells.
The very virulent infectious bursal disease virus (vvIBDV) Gx strain causes over 60% mortality in chickens but cannot replicate in CEF cultures.
In vitro growth properties of recombinant virus were also inspected and concluded that the MZC12HA/NA had the same growth kinetics in CEF cultures as its parental wild type virus GX0101.
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The results showed that any one of these three adipogenic transcription factors was sufficient to trigger the adipogenic program in CEFs, as demonstrated by accumulation of cytoplasmic lipid droplets and expression of the adipocyte marker gene (adipocyte fatty acid binding protein, A-FABP).
This is important because, in my experience, past performance is the best indicator of future results in CEF-land.
DF-1 cells are IFN competent [34] and plasmid transfection induces IFN in CEFs [35].
rs3885907, which was most significantly associated in CEF-treated patients, was located in an intron of ALOX5AP.
The percentage of ciliary extension in CEFs was measured in at least 20 microscopic fields.
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